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Additional Guiltinan Lab Protocols

under development, please email if you want a specific protocol

Current Working Protocols

  1. Agrobacterium
    1. Agrotransformation
    2. Agrobacterium Miniscreen
    3. Electroporation of Agrobacterium
    4. Preparation of electrocompetent Agrobacterium
  2. Chitinase Assay
    1. Complete endochitinase protocol
  3. DNA Protocols
    1. Construction of a full-length cDNA library of Theobroma cocao
    2. DNA Preps - Ann
    3. Blunt end ligations with Promega T4 DNA Polymerase
    4. Blunt end ligations with New England Biological T4 DNA Polymerase
    5. Cesium Chloride DNA Preparation
    6. Cloning protocol
    7. Dephosphorylation using Calf Intestinal Phosphatase (CIP)
    8. Extraction of Genomic DNA from Theobroma cocao
    9. Inverted repeats cloning protocol
    10. DNA Preps and Cloning – EVE
    11. DNA Prep for PCR using N-Amp
    12. Ligation to pGem vector
    13. DNA Purification from Gel using GENECLEAN
    14. Plasmid Extraction from E. coli
    15. The Sucrose Prep for PCR for Arabidopsis
    16. Transformation to E. coli
    17. Hybridization Protocol for BAC Colony Filters
    18. PCR DNA Extraction 
    19. Plant Preps
    20. Dellaporta DNA Extraction Midi Prep
    21. 96-well Edwards Prep
    22. Plant DNA Microprep protocol
  4. Maize Specific Protocols
  5. Starch Prep-Dinges
  6. SBE in vitro activity assay D Fisher
  7. SBE Radioactive Phospha Laurens Assay
  8. Maize Greenhouse growth
  9. Corn Fertilizer Program 
  10. Zymograms
  11. Native Zymograms – Laurens
  12. Zymogram Protocol - Nelson
  13. Proteins
    1. Western analysis – Blauth
    2. Native Western – Nelson
    3. Taq Polymerase Purification
  14. RNA
    1. DEPC treated water
    2. Northern blot protocol
    3. RNA Extraction Protocol – Cocoa
    4. RNA Sampling with RNAlater – cocoa
    5. RNA later preparation
  15. Transformation Protocols
    1. Cacao
    2. Arabidopsis
    3. Tobacco
    4. Apple

Alternative Protocols


16.  DNA tricks

17.  4-way ligation

18.  DNA fragment isolation

19.  Probes, electroelute + Label

20.  Annealing Oligo’s for probe

21.  T4 DNA polymerase treatment and ligation protocol

22.  DNA Binding proteins

23.  Crude nuclear extraction

24.  DNA binding and EMSA

25.  Gel Shift protocol

26.  Linda’s protocols

27.  Down Under Gels

28.  High Ionic Strength EMSA

29.  In vitro transcription

30.  Isolation of ABRE fragment

31.  Primers of PCR expression TnT

32.  Tricine glycine gels

33.  Mini Nuclear Extraction

34.  Nucleosome Reconstitution Protocol

35.  Nuclear Protein Extract of Wheat Embryos

36.  Southwestern Blot

37.  Tag Team Primer and PCR Tips

38.  Electroporation

  1. Cleaning electroporation cuvettes
  2. E. coli electroporation

39.  Gene Gun

  1. Gene Gun Protocol

40.  GFP Methods

  1. GFP detection

41.  Maize

  1. DNA Extraction-Dellaporta (old)
  2. Kernal Starch extraction
  3. Leaf – SBE and starch extraction
  4. SBE protein assay
  5. SBE.Mu genotyping 6.1.99

42.  Phosphorimager

  1. Phosphorimager Tips

43.  RNA Tricks

  1. Cleaning RNA
  2. Older Northern protocol
  3. Northern Blot (Older)
  4. Northern Hybridization
  5. RNA Isolation from tobacco

44.  Untested Protocols

  1. Prostain Fluorescent Protocol

45.   Useful Reference documents

  1. Cloning into pBI121
  2. History of pMON strains
  3. History of agro strains
  4. Electroporation
  5. Agro History