Characterization of circadian-regulated mRNAs encoding glycine-rich RNA-binding proteins in Pelargonium x hortorum


Clark, D.G.; Richards, C.; Brown, K.M.


Physiologia Plantarum, Volume 106, Issue 4, p.409-414 (1999)

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Two cDNAs, PhGRP1 and PhGRP2, with high sequence identity to genes encoding glycine-rich RNA-binding proteins, were cloned from Pelargonium hortorum. PhGRP is represented in the P. hortorum genome by a single copy. DNA sequence analysis of PhGRP1 and PhGRP2 indicated that PhGRP2 represents a partially spliced version of PhGRP1. The additional intron-like sequence in PhGRP2 contains a termination codon, which would result in translation of a truncated protein. Expression of PhGRP1 was high in petals, pistils, roots and stems, and lower in leaves. PhGRP2 transcripts were less abundant than those of PhGRP1 in all tissues, but were proportionately higher in flower tissues than in roots or stems, and were not detectable in leaves. PhGRP transcripts were highest in stigma and style tissues, but were unaffected by exogenous ethylene treatment or pollination. PhGRP transcript levels fluctuated in a circadian rhythm with maximal accumulation late in the day. The nucleotide sequence data reported herre appear in the EMBL, GenBank and DDBJ databases under accession numbers AF009003 and AF009004