Analysis results can be sensitive to the threshold parameters used. WinRhizo can automatically set these, but you may want to manually tweak them from time to time. These analysed images are from the root image previously shown, but are a selection of a smaller region for clarity. The color traces on the root indicate where roots have been detected.

Poor Threshold Value Proper Threshold value

Image 1. the majority of the fine roots have not been detected. Total Root length (whole sample) was 77.63 cm
Image 2. with the proper threshold setting, root detection is nearly complete, and the Total Root length was 332.43 cm