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Department of Plant Science

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Guiltinan-Maximova Lab
Protocols
Photographs of Cacao Tissue Culture Protocols
Dissecting Cacao Flowers for Somatic Embryogenesis

Dissecting Cacao Flowers for Somatic Embryogenesis

Unopened Cacao Flowers

Placed in Sterile 50ml Tube

Adding Sterilization Solution

Incubation in Sterilization Solution

(inverted)

Decant Sterilization Solution

Rinse with Sterile Water

Place in Sterile Petri Dish

Hold Gently with Forceps

Cut Near Base with Scalpel

Remove Base

Remove Petals, Stamens, and Staminodes

Place Petals, Stamens, and Staminodes on Fresh Media

Separate Flower Parts and Distribute on Petri Dish

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