Unopened Cacao Flowers
Placed in Sterile 50ml Tube
Adding Sterilization Solution
Incubation in Sterilization Solution
(inverted)
Decant Sterilization Solution
Rinse with Sterile Water
Place in Sterile Petri Dish
Hold Gently with Forceps
Cut Near Base with Scalpel
Remove Base
Remove Petals, Stamens, and Staminodes
Place Petals, Stamens, and Staminodes on Fresh Media
Separate Flower Parts and Distribute on Petri Dish